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KMID : 0363320060270010149
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Journal of Korean Oriental Internal Medicine
2006 Volume.27 No. 1 p.149 ~ p.165
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The Effects of Saengkankunbi-tang on Proliferation, Apoptosis and Cell Signaling Pathways of HepG2 Cells
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Kim Jae-Yong
Kim Young-Chul
Lee Jang-Hoon
Woo Hong-Jung
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Abstract
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Objectives: This study was done to evaluate the effects of Saengkankunbi-tang on cell-viability, proliferation, cell-cycle, apoptosis and DNA replication on HepG2 cell and to find out by which molecular-biological mechanism by which Saengkankunbi-tang operates.
Methods : The MTT assay, cell counting assay, [3H]-thymidine incorporation assay, flow cytometric analysis, tryphan blue exclusion assay, western blot analysis, quantative RT-PCR were taken.
Results : Saengkankunbi-tang had no effect on proliferation, cell-cycle and DNA replications of HepG2 cells, while it improved cell viability and reduced apoptosis, and it activated Akt and NFKB. But, it did not produce an effect on cell viability and apoptosis when P13K/Akt pathway was blocked by LY294002 nor when NF{kapa}B activation was blocked by DN-I{kapa}B.
Conclusion : These results suggests that Saengkankunbi-tang improves cell viability and reduces apoptosis of HepG2 cells, by activating NF{kapa}B through PI3K/Akt pathway.
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KEYWORD
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Saengkankunbi-tang, PI3K/Akt pathway, NF{kapa}B, apoptosis, HepG2 cell
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